tailieunhanh - o cáo hóa học:" Bisphosphonate-associated osteonecrosis of the jaw is linked to suppressed TGFb1-signaling and increased "

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Bisphosphonate-associated osteonecrosis of the jaw is linked to suppressed TGFb1-signaling and increased | Wehrhan et al. Journal of Translational Medicine 2011 9 102 http content 9 1 102 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Bisphosphonate-associated osteonecrosis of the jaw is linked to suppressed TGFp1-signaling and increased Galectin-3 expression A histological study on biopsies 1 2 3 1 1 1 Falk Wehrhan Peter Hyckel Arndt Guentsch Emeka Nkenke Phillip Stockmann Karl A Schlegel Friedrich W Neukam1 and Kerstin Amann4 Abstract Background Bisphosphonate associated osteonecrosis of the jaw BRONJ implies an impairment in oral hard- and soft tissue repair. An understanding of the signal transduction alterations involved can inform therapeutic strategies. Transforming growth factor b1 TGFb1 is a critical regulator of tissue repair galectin-3 mediates tissue differentiation and specifically modulates periodontopathic bacterial infection. The aim of this study was to compare the expression of TGFb1-related signaling molecules and Galectin-3 in BRONJ-affected and healthy mucosal tissues. To discriminate between BRONJ-specific impairments in TGFb1 signaling and secondary inflammatory changes the results were compared to the expression of TGFb1 and Galectin-3 in mucosal tissues with osteoradionecrosis. Methods Oral mucosal tissue samples with histologically-confirmed BRONJ n 20 osteoradionecrosis n 20 and no lesions normal n 20 were processed for immunohistochemistry. Automated staining with an alkaline phosphatase-anti-alkaline phosphatase kit was used to detect TGFP1 Smad-2 3 Smad-7 and Galectin-3. We semiquantitatively assessed the ratio of stained cells total number of cells labeling index Bonferroni-adjustment . Results TGFP1 and Smad-2 3 were significantly decreased p and p respectively in the BRONJ samples and significantly increased p and p respectively in the osteoradionecrosis samples compared to normal tissue. Smad-7 was significantly increased p in the BRONJ group and significantly decreased