tailieunhanh - báo cáo hóa học:" Plasma proteome changes in cardiovascular disease patients: novel isoforms of apolipoprotein A1"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Plasma proteome changes in cardiovascular disease patients: novel isoforms of apolipoprotein A1 | Májek et al. Journal of Translational Medicine 2011 9 84 http content 9 1 84 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Plasma proteome changes in cardiovascular disease patients novel isoforms of apolipoprotein A1 1 112 2 1 1 Pavel Májek Zuzana Reicheltová Jiri Suttnar Martin Malý Milan Oravec Klára Pecánková and Jan E Dyr Abstract Background The aim of this proteomic study was to look for changes taking place in plasma proteomes of patients with acute myocardial infarction AMI unstable angina pectoris UAP and stable angina pectoris SAP . Methods Depleted plasma proteins were separated by 2D SDS-PAGE pl 4-7 and proteomes were compared using Progenesis SameSpots statistical software. Proteins were identified by nanoLC-MS MS. Proteins were quantified using commercial kits. Apolipoprotein A1 was studied using 1D and 2D SDS-PAGE together with western blotting. Results Reciprocal comparison revealed 46 unique significantly different spots proteins in 34 spots were successfully identified and corresponded to 38 different proteins. Discrete comparisons of patient groups showed 45 41 and 8 significantly different spots when AMI UAP and SAP were compared with the control group. On the basis of our proteomic data plasma levels of two of them alpha-1 microglobulin and vitamin D-binding protein were determined. The data however failed to prove the proteins to be suitable markers or risk factors in the studied groups. The plasma level and isoform representation of apolipoprotein A1 were also estimated. Using 1D and 2D SDS-PAGE together with western blotting we observed extra high-molecular weight apolipoprotein A1 fractions presented only in the patient groups indicating that the novel high-molecular weight isoforms of apolipoprotein A1 may be potential new markers or possible risk factors of cardiovascular disease. Conclusion The reported data show plasma proteome changes in patients with AMI UAP and SAP. We propose some .