tailieunhanh - báo cáo hóa học:" Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets | Salaun et al. Journal of Translational Medicine 2011 9 44 http content 9 1 44 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Differentiation associated regulation of microRNA expression in vivo in human CD8 T cell subsets D ri I c - I - I I 1 f I -x 1 1 r x -X m X m z f z 2f D -X r- r- -X rvx D X I r X 3 4 r I I lzz I r I I z-xt- r I I z i I I V Izz-xt- 2 A hex I S-. D I I X 5 I I I l í r D xÌ4 r z l k1 Bruno Sdldun idKuyd Idmdmoio Bdssdm Bddrdn YdSUKo isuneisugu-iOKoid Antoine Roux LUKdS Bdltscn Redoudne Rouds3 Hussein Fayyad-Kazan3 Petra Bdumgdertner1 Estelle Devevre1 Anirudn Rdmesh6 115 3 5 1 Mdrion Braun Ddniel Speiser Brigitte Autrdn Philippe Mdrtidt Victor Appdy 1 dnd Pedro Romero 1 Abstract Background The differentidtion of CD8 T lymphocytes following priming of ndive cells is central in the estdblisnment of the dddptive immune response. Yet the moleculdr events underlying this process dre not fully understood. MicroRNAs hdve been recently shown to pldy d Key role in the reguldtion of hdemdtopoiesis in mouse but their implicdtion in peripheral lymphocyte differentidtion in humdns remdins ldrgely unKnown. Methods In order to explore the potentidl implicdtion of microRNAs in CD8 T cell differentidtion in humdns microRNA expression profiles were dndlysed using microdrrdys dnd qudntitdtive PCR in several humdn CD8 T cell subsets defining the mdjor steps of the T cell differentidtion pdthwdy. Results We found expression of d limited set of microRNAs including the miR-17 92 cluster. Moreover we revedl the existence of differentidtion-dssocidted reguldtion of specific microRNAs. When compdred to ndive cells miR-21 dnd miR-155 were indeed found upreguldted upon differentidtion to effector cells while expression of the miR-17 92 cluster tended to concomitdntly decredse. Conclusions This study estdblishes for the first time in d ldrge pdnel of individudls the existence of differentidtion dssocidted reguldtion of microRNA .