tailieunhanh - Báo cáo toán học: "nfliximab and Etanercept Are Equally Effective in Reducing Enterocyte APOPTOSIS in Experimental Colitis"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Infliximab and Etanercept Are Equally Effective in Reducing Enterocyte APOPTOSIS in Experimental Colitis | Int. J. Med. Sci. 2008 5 169 International Journal of Medical Sciences ISSN 1449-1907 2008 5 4 169-180 Ivyspring International Publisher. All rights reserved Research Paper Infliximab and Etanercept Are Equally Effective in Reducing Enterocyte APOPTOSIS in Experimental Colitis Walter Fries1 Carmelo Muja2 Carmela Crisafulli2 Giuseppe Costantino1 Giuseppe Longo1 Salvatore Cuzzocrea2 3 Emanuela Mazzon2 3 1. Dipartimento di Medicina Interna e Terapia Medica Sezione di Farmacologia Università di Messina Messina Italy. 2. Dipartimento Clinico-Sperimentale di Medicina e Farmacologia Sezione di Farmacologia Università di Messina Messina Italy. 3. IRCCS Centro Neurolesi Bonino-Pulejo Messina Italy. Correspondence to Walter Fries MD Dipartimento di Medicina Interna e Terapia Medica Policlinico Universitario Pad C - III piano Via Consolare Valeria 1 98100 Messina - Italy. Tel. 39 090 2212373 fax 39 090 2935162 e-mail fwalter@ Received Accepted Published Loss of epithelial barrier integrity is considered an early step in the pathogenesis of Crohn s disease CD and the rate of enterocyte apoptosis is one of the determinants of the intestinal barrier function. Tumor necrosis factor-a TNF-a one of the major proinflammatory mediators in CD is one of the extrinsic signals which initiate apoptosis of enterocytes. The aim of this study was to investigate the early effects of experimental colitis on enterocyte apoptosis and the effects of two anti-TNF treatments infliximab IFX and etanercept ETC . In addition the importance of receptor I for TNF was tested in TNFR-1- - mice. Circulating TNF-a levels were effectively reduced by IFX and ETC p both at 3 and 6 h. Apoptosis of the ileal enterocytes assessed by TUNEL staining staining for Fas-ligand and bax increased at 3 and 6h. These alterations were prevented by both anti-TNF strategies and in TNFR-17- animals. The anti-apoptotic protein Bcl-2 was expressed in the ileal .