tailieunhanh - Báo cáo toán học: "Differential display identifies overexpression of the USP36 gene, encoding a deubiquitinating enzyme, in ovarian cancer"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Differential display identifies overexpression of the USP36 gene, encoding a deubiquitinating enzyme, in ovarian cancer. | Int. J. Med. Sci. 2008 5 133 Research Paper International Journal of Medical Sciences ISSN 1449-1907 2008 5 3 133-142 Ivyspring International Publisher. All rights reserved Differential display identifies overexpression of the USP36 gene encoding a deubiquitinating enzyme in ovarian cancer Jianduan Li1 Lisa M. Olson1 2 Zhengyan Zhang1 Lina Li1 3 Miri Bidder1 Loan Nguyen1 John Pfeifer4 Janet S. Rader1 1. Department of Obstetrics and Gynecology Division of Gynecologic Oncology Washington University School of Medicine St. Louis MO 63110 USA 2. Abbott Bioresearch Center Worcester MA USA 3. Laboratory of Biochemical Genetics National Heart Lung and Blood Institute NIH Bethesda USA 4. Department of Pathology Washington University School of Medicine St. Louis MO 63110 USA Correspondence to Janet S. Rader MD Professor Department of Obstetrics and Gynecology Division of Gynecologic Oncology Department of Genetics Washington University School of Medicine Box 8064 4911 Barnes-Jewish Hospital Plaza St. Louis MO 63110. Phone 314-362-3181 fax 314-362-2893 email raderj@ Received Accepted Published Objectives. To find potential diagnostic markers or therapeutic targets we used differential display technique to identify genes that are over or under expressed in human ovarian cancer. Methods. Genes were initially identified by differential display between two human ovarian surface epithelium cultures and two ovarian cancer cell lines A2780 and Caov-3. Genes were validated by relative quantitative RT-PCR and RNA in situ hybridization. Results. Twenty-eight non-redundant sequences were expressed differentially in the normal ovarian epithelium and ovarian cancer cell lines. Seven of the 28 sequences showed differential expression between normal ovary and ovarian cancer tissue by RT-PCR. USP36 was over-expressed in ovarian cancer cell lines and tissues by RT-PCR and RNA in situ hybridization. Northern blot analysis and RT-PCR .