tailieunhanh - Báo cáo hóa học: " Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA | Virology Journal BioMed Central Open Access Short report Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA Wang Pengyan Ren Yan Guo Zhiru and Chen Chuangfu Address College of Animal Science and Technology Shihezi University Shihezi Xinjiang 832003 PR China Email WangPengyan-wwwpy_322@ RenYan-rycb1225@ Guo Zhiru -guozhiru@ Chen Chuangfu - ccf-xb@ Corresponding authors Published 25 July 2008 Received 23 April 2008 Virology Journal 2008 5 86 doi 1743-422X-5-86 Accepted 25 July 2008 This article is available from http content 5 1 86 2008 Pengyan et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ By using bioinformatics computer programs all foot-and-mouth disease virus FMDV genome sequences in public-domain databases were analyzed. Based on the results of homology analysis 2 specific small interfering RNA siRNA targeting homogenous 3D and 2B1 regions of 7 serotypes of FMDV were prepared and 2 siRNA-expression vectors pSi-FMD2 and pSi-FMD3 were constructed. The siRNA-expressing vectors were used to test the ability of siRNAs to inhibit virus replication in baby hamster kidney BHK-21 cells and suckling mice a commonly used small animal model. The results demonstrated that transfection of BHK-21 cells with siRNA-expressing plasmids significantly weakened the cytopathic effect CPE . Moreover BHK-21 cells transiently transfected with short hairpin RNA shRNA -expressing plasmids were specifically resistant to the infection of the FMDV serotypes A O and Asia I and this the antiviral effects persisted for almost 48 hours. We measured the viral .

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