tailieunhanh - Báo cáo hóa học: " HIV-1 designed to use different tRNAGln isoacceptors prefers to select tRNAThr for replication"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: HIV-1 designed to use different tRNAGln isoacceptors prefers to select tRNAThr for replication | Virology Journal BioMed Central Research Open Access HIV-1 designed to use different tRNAGln isoacceptors prefers to select tRNAThr for replication Meng Li Peter G Eipers Na Ni and Casey D Morrow Address Department of Cell Biology University of Alabama at Birmingham 35294-0024 Birmingham AL USA Email Meng Li - prisslimli@ Peter G Eipers - peipers@ Na Ni - niinaa1@ Casey D Morrow - caseym@ Corresponding author Published 26 September 2006 Received 19 September 2006 Accepted 26 September 2006 Virology Journal 2006 3 80 doi 186 1743-422X-3-80 This article is available from http content 3 1 80 2006 Li et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Previous studies have shown that infection with human immunodeficiency virus type 1 HIV-1 causes acceleration of the synthesis of glutamine tRNA tRNAGln in infected cells. To investigate whether this might influence HIV-1 to utilize tRNAGln as a primer for initiation of reverse transcription we have constructed HIV-1 proviral genomes in which the PBS and the A-loop region upstream of the PBS have been made complementary to either the anticodon region of tRNAGln 1 or tRNAGln 3 and 3 terminal 18 nucleotides of each isoacceptor of tRNAGln. Results Viruses in which the PBS was altered to be complementary to tRNAGlni1 or tRNAGln 3 with or without the A-loop all exhibited a lower infectivity than the wild type virus. Viruses with only the PBS complementary to tRNAGln 1 or tRNAGln 3 reverted to wild type following culture in SupT1 cells. Surprisingly viruses in which the PBS and A-loop were complementary to tRNAGln 1 did not grow in SupT1 cells while viruses in which the PBS and A-loop were made complementary to .