tailieunhanh - Báo cáo hóa học: " Quantitative estimation of Nipah virus replication kinetics in vitro"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Quantitative estimation of Nipah virus replication kinetics in vitro | Virology Journal BioMed Central Research Quantitative estimation of Nipah virus replication kinetics in vitro Li-Yen Chang1 AR Mohd Ali2 Sharifah Syed Hassan2 and Sazaly AbuBakar 3 Open Access Address 1Center for Proteomics Research Department of Forest Biotechnology Forest Research Institute 52109 Selangor Malaysia Veterinary Research Institute Jalan Sultan Azlan Shah 13800 Ipoh Perak Malaysia and Department of Medical Microbiology Faculty of Medicine University Malaya 50603 Kuala Lumpur Malaysia Email Li-Yen Chang - changliyen@ AR Mohd Ali - ali@ Sharifah Syed Hassan - sharifas@ Sazaly AbuBakar - sazaly@ Corresponding author Published 19 June 2006 Received 16 January 2006 Accepted 19 June 2006 Virologyjournal 2006 3 47 doi I86 I743-422X-3-47 This article is available from http content 3 1 47 2006 Chang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Background Nipah virus is a zoonotic virus isolated from an outbreak in Malaysia in 1 998. The virus causes infections in humans pigs and several other domestic animals. It has also been isolated from fruit bats. The pathogenesis of Nipah virus infection is still not well described. In the present study Nipah virus replication kinetics were estimated from infection of African green monkey kidney cells Vero using the one-step SYBR Green I-based quantitative real-time reverse transcriptase-polymerase chain reaction qRT-PCR assay. Results The qRT-PCR had a dynamic range of at least seven orders of magnitude and can detect Nipah virus from as low as one PFU pL. Following initiation of infection it was estimated that