tailieunhanh - Báo cáo hóa học: " Natural products that reduce rotavirus infectivity identified by a cell-based moderate-throughput screening assay"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Natural products that reduce rotavirus infectivity identified by a cell-based moderate-throughput screening assay | Virology Journal BioMed Central Open Access Methodology Natural products that reduce rotavirus infectivity identified by a cell-based moderate-throughput screening assay Mark E Shaneyfelt Anna D Burke Joel W Graff Mark A Jutila and Michele E Hardy Address Veterinary Molecular Biology Montana State University Bozeman MT 59715 USA Email Mark E Shaneyfelt - mshaneyf@ Anna D Burke - annaburke@ Joel W Graff-jgraff@ Mark A Jutila - uvsmj@ Michele E Hardy - mhardy@ Corresponding author Published 01 September 2006 Received 22 June 2006 Accepted 01 September 2006 Virology journal 2006 3 68 doi l743-422X-3-68 This article is available from http content 3 1 68 2006 Shaneyfelt et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background There is widespread interest in the use of innate immune modulators as a defense strategy against infectious pathogens. Using rotavirus as a model system we developed a cell-based moderate-throughput screening MTS assay to identify compounds that reduce rotavirus infectivity in vitro toward a long-term goal of discovering immunomodulatory agents that enhance innate responses to viral infection. Results A natural product library consisting of 280 compounds was screened in the assay and 15 compounds that significantly reduced infectivity without cytotoxicity were identified. Time course analysis of four compounds with previously characterized effects on inflammatory gene expression inhibited replication with pre-treatment times as minimal as 2 hours. Two of these four compounds a-mangostin and 18-P-glycyrrhetinic acid activated .