tailieunhanh - Báo cáo hóa học: " VPg of murine norovirus binds translation initiation factors in infected cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: VPg of murine norovirus binds translation initiation factors in infected cells | Virology Journal BioMed Central Research Open Access VPg of murine norovirus binds translation initiation factors in infected cells Katie F Daughenbaugh1 Christiane E Wobus2 and Michele E Hardy 1 Address Veterinary Molecular Biology Montana State University Bozeman MT USA and 2Department of Pathology and Immunology Washington University School of Medicine St Louis MO USA Email Katie F Daughenbaugh - katief@ Christiane E Wobus - cwobus@ Michele E Hardy - mhardy@ Corresponding author Published 23 May 2006 Received 06 March 2006 Accepted 23 May 2006 Virology Journal 2006 3 33 doi 186 1743-422X-3-33 This article is available from http content 3 1 33 2006 Daughenbaugh et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Norovirus genomic and subgenomic RNAs are covalently linked at the 5 nucleotide to a 15 kD protein called VPg. VPg of two human norovirus strains binds translation initiation factor eIF3 and other eIFs in vitro suggesting VPg functions in initiation of protein synthesis on viral RNA. Human norovirus strains are not cultivable and thus experimental evidence of interactions between VPg and eIFs in infected cells has been lacking. We used the cultivable murine norovirus MNV-1 as a model to study interactions between VPg and eIFs in infected cells. Results As shown previously for human norovirus VPg MNV-1 VPg bound eIF3 eIF4GI eIF4E and S6 ribosomal protein in cell extracts by GST pull-down assay. Importantly MNV-l VPg coprecipitated eIF4GI and eIF4E from infected macrophages providing evidence that VPg interacts with components of the translation machinery in norovirus infected cells. Conclusion The interactions between MNV-1 VPg and eIFs .