tailieunhanh - Báo cáo hóa học: " Temperature sensitive influenza A virus genome replication results from low thermal stability of polymerase-cRNA complexes"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Temperature sensitive influenza A virus genome replication results from low thermal stability of polymerase-cRNA complexes | BioMed Central Virology Journal Research Temperature sensitive influenza A virus genome replication results from low thermal stability of polymerase-cRNA complexes Rosa M Dalton1 Anne E Mullin1 Maria Joao Amorim1 Elizabeth Medcalf1 Laurence S Tiley2 and Paul Digard 1 Open Access Address Division of Virology Department of Pathology University of Cambridge Tennis Court Road Cambridge CB2 1QP UK and 2Centre for Veterinary Science University of Cambridge Madingley Road Cambridge CB3 OES UK Email Rosa M Dalton - rmd39@ Anne E Mullin - anne@ Maria Joao Amorim - mjlgda2@ Elizabeth Medcalf - eam27@ Laurence S Tiley - Lst21@ Paul Digard - pd1@ Corresponding author Published 25 August 2006 Received 16 June 2006 Accepted 25 August 2006 Virologyjournal 2006 3 58 doi I86 I743-422X-3-58 This article is available from http content 3 1 58 2006 Dalton et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The RNA-dependent RNA polymerase of Influenza A virus is a determinant of viral pathogenicity and host range that is responsible for transcribing and replicating the negative sense segmented viral genome vRNA . Transcription produces capped and polyadenylated mRNAs whereas genome replication involves the synthesis of an alternative plus-sense transcript cRNA with unmodified termini that is copied back to vRNA. Viral mRNA transcription predominates at early stages of viral infection while later negative sense genome replication is favoured. However the switch that regulates the transition from transcription to replication is poorly understood. Results We show that temperature strongly affects the balance between