tailieunhanh - Báo cáo hóa học: " Membrane-associated heparan sulfate is not required for rAAV-2 infection of human respiratory epithelia"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Membrane-associated heparan sulfate is not required for rAAV-2 infection of human respiratory epithelia | Virology Journal BioMed Central Research Open Access Membrane-associated heparan sulfate is not required for rAAV-2 infection of human respiratory epithelia Michael P Boyle 1 Raymond A Enke1 Jeffrey B Reynolds1 Peter J Mogayzel Jr2 William B Guggino2 3 and Pamela L Zeitlin2 Address Department of Medicine The Johns Hopkins University School of Medicine Baltimore MD 21205 USA 2Department of Pediatrics The Johns Hopkins University School of Medicine Baltimore MD 21205 USA and 3Department of Physiology The Johns Hopkins University School of Medicine Baltimore MD 21205 USA Email Michael P Boyle - mboyle@ Raymond A Enke - renke@ Jeffrey B Reynolds - jreynolds@ Peter J Mogayzel - pmogayze@ William B Guggino - wguggino@ Pamela L Zeitlin - pzeitlin@ Corresponding author Published 22 April 2006 Received 27 October 2005 Accepted 22 April 2006 VirologyJournal2006 3 29 doi l743-422X-3-29 This article is available from http content 3 1 29 2006Boyle et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Adeno-associated virus type 2 AAV-2 attachment and internalization is thought to be mediated by host cell membrane-associated heparan sulfate proteoglycans HSPG . Lack of HSPG on the apical membrane of respiratory epithelial cells has been identified as a reason for inefficient rAAV-2 infection in pulmonary applications in-vivo. The aim of this investigation was to determine the necessity of cell membrane HSPG for efficient infection by rAAV-2. Results Rates of transduction with rAAV2-CMV-EGFP3 in several different immortalized airway epithelial cell lines were determined at different multiplicities of infection MOI before and after removal of .