tailieunhanh - Báo cáo hóa học: " Amphotropic murine leukemia virus is preferentially attached to cholesterol-rich microdomains after binding to mouse fibroblasts"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Amphotropic murine leukemia virus is preferentially attached to cholesterol-rich microdomains after binding to mouse fibroblasts | Virology Journal BioMed Central Research Open Access Amphotropic murine leukemia virus is preferentially attached to cholesterol-rich microdomains after binding to mouse fibroblasts Christiane Beer and Lene Pedersen Address Institute of Clinical Medicine and Department of Molecular Biology University of Aarhus Aarhus Denmark Email Christiane Beer - chb@ Lene Pedersen - lp@ Corresponding author Published 02 April 2006 Received 03 November 2005 Accepted 02 April 2006 Virology Journal 2006 3 21 doi 1743-422X-3-21 This article is available from http content 3 1 21 2006 Beer and Pedersen licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background We have recently shown that amphotropic murine leukemia virus A-MLV can enter the mouse fibroblast cell line NIH3T3 via caveola-dependent endocytosis. But due to the size and omega-like shape of caveolae it is possible that A-MLV initially binds cells outside of caveolae. Rafts have been suggested to be pre-caveolae and we here investigate whether A-MLV initially binds to its receptor Pit2 a sodium-dependent phosphate transporter in rafts or caveolae or outside these cholesterol-rich microdomains. Results Here we show that a high amount of cell-bound A-MLV was attached to large rafts of NIH3T3 at the time of investigation. These large rafts were not enriched in caveolin-l a major structural component of caveolae. In addition they are rather of natural occurrence in NIH3T3 cells than a result of patching of smaller rafts by A-MLV. Thus cells incubated in parallel with vesicular stomatitis virus glycoprotein VSV-G pseudotyped MLV particles showed the same pattern of large rafts as cells incubated with A-MLV but VSV-G pseudotyped MLV .