tailieunhanh - Báo cáo hóa học: " A novel cloning strategy for isolating, genotyping and phenotyping genetic variants of geminiviruses"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: A novel cloning strategy for isolating, genotyping and phenotyping genetic variants of geminiviruses | Virology Journal BioMed Central Open Access Methodology A novel cloning strategy for isolating genotyping and phenotyping genetic variants of geminiviruses Cica Urbino 1 Gael Thébaud2 Martine Granier1 Stéphane Blanc2 and Michel Peterschmitt1 Address 1CIRAD-UMR BGPI F-34398 Montpellier France and 2INRA-UMR BGPI F-34398 Montpellier France Email Cica Urbino - Gael Thébaud - Martine Granier - Stéphane Blanc - Michel Peterschmitt - Corresponding author Published 31 October 2008 Received 25 September 2008 __ AAAO J .IA I loAiiTAt A- - v r Ilf Accepted 31 October 2008 Virology Journal 2008 5 135 doi l743-422X-5-l35 This article is available from http content 5 l l35 2008 Urbino et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Viruses of the genus Begomovirus Geminiviridae are emerging economically important plant viruses with a circular single-stranded DNA genome. Previous studies have shown that geminiviruses and RNA viruses exhibit similar mutation frequencies although geminiviruses are replicated by host DNA polymerases and RNA viruses by their own virus-encoded error-prone RNA-dependent RNA-polymerase. However the phenotypic effects of naturally occurring mutations have never been extensively investigated in geminiviruses particularly because to be infectious cloned viral genomes usually require sub-cloning as complete or partial tandem repeats into a binary vector from Agrobacterium tumefaciens. Results Using Tomato yellow leaf curl virus TYLCV we show