tailieunhanh - Báo cáo hóa học: " Adaptation to cell culture induces functional differences in measles virus proteins"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Adaptation to cell culture induces functional differences in measles virus proteins | Virology Journal BioMed Central Research Adaptation to cell culture induces functional differences in measles virus proteins Bettina Bankamp 1 Judith M Fontana2 William J Bellini1 and Paul A Rota1 Open Access Address 1Measles Mumps Rubella and Herpesvirus Laboratory Branch Division of Viral Diseases Centers for Disease Control and Prevention MS C-22 1600 Clifton Road Atlanta Georgia 30333 USA and 2Department of Microbiology and Immunology Uniformed Services University of the Health Sciences 4301 Jones Bridge Road Bethesda Maryland 20814 USA Email Bettina Bankamp - bbankamp@ Judith M Fontana - William J Bellini - wbellini@ Paul A Rota - prota@ Corresponding author Published 27 October 2008 Received 25 September 2008 _ J .IA I loAiiTAt A- - v r no Accepted 27 October 2008 Virology Journal 2008 5 129 doi 1743-422X-5-129 This article is available from http content 5 1 129 2008 Bankamp et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background Live attenuated measles virus MeV vaccine strains were generated by adaptation to cell culture. The genetic basis for the attenuation of the vaccine strains is unknown. We previously reported that adaptation of a pathogenic wild-type MeV to Vero cells or primary chicken embryo fibroblasts CEFs resulted in a loss of pathogenicity in rhesus macaques. The CEF-adapted virus D-CEF contained single amino acid changes in the C and matrix M proteins and two substitutions in the shared amino terminal domain of the phosphoprotein P and V protein. The Vero-adapted virus D-VI had a mutation in the cytoplasmic tail of the .