tailieunhanh - Báo cáo hóa học: " Detection and characterization of chicken anemia virus from commercial broiler breeder chickens"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Detection and characterization of chicken anemia virus from commercial broiler breeder chickens | Virology Journal BioMed Central Open Access Detection and characterization of chicken anemia virus from commercial broiler breeder chickens Zerihun Hailemariam1 3 Abdul Rahman Omar 2 3 Mohd Hair-Bejo3 and Tan Ching Giap3 Address faculty of Veterinary Medicine Haramaya University . Box 271 Haramaya Ethiopia institute of BioScience Universiti Putra Malaysia 43400 UPM Serdang Selangor Darul Ehsan Malaysia and 3Faculty of Veterinary Medicine Universiti Putra Malaysia 43400 UPM Serdang Selangor Darul Ehsan Malaysia Email Zerihun Hailemariam - zerishh@ Abdul Rahman Omar - aro@ Mohd Hair- Bejo - mdhair@ Tan Ching Giap - barney_tan@ Corresponding author Published 27 October 2008 Received 2 September 2008 Accepted 27 October 2008 Virology Journal 2008 5 128 doi 1743-422X-5-128 This article is available from http content 5 1 128 2008 Hailemariam et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Chicken anemia virus CAV is the causative agent of chicken infectious anemia CIA . Study on the type of CAV isolates present and their genetic diversity transmission to their progeny and level of protection afforded in the breeder farms is lacking in Malaysia. Hence the present study was aimed to detect CAV from commercial broiler breeder farms and characterize CAV positive samples based on sequence and phylogenetic analysis of partial VP1 gene. Results A total of 12 CAV isolates from different commercial broiler breeder farms were isolated and characterized. Detection of CAV positive embryos by the PCR assay in the range of 40 to 100 for different farms indicated high level of occurrence of vertical transmission of viral DNA to the progeny. .