tailieunhanh - Báo cáo hóa học: " Recombination in feline immunodeficiency virus from feral and companion domestic cats"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Recombination in feline immunodeficiency virus from feral and companion domestic cats | Virology Journal BioMed Central Research Recombination in feline immunodeficiency virus from feral and companion domestic cats Jessica J Hayward and Allen G Rodrigo Open Access Address Bioinformatics Institute Allan Wilson Centre for Molecular Ecology and Evolution School of Biological Sciences The University of Auckland Auckland New Zealand Email Jessica J Hayward - Allen G Rodrigo - Corresponding author Published 17 June 2008 Received 25 April 2008 Accepted 17 June 2008 Virology Journal 2008 5 76 doi 186 1743-422X-5-76 This article is available from http content 5 1 76 2008 Hayward and Rodrigo licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Recombination is a relatively common phenomenon in retroviruses. We investigated recombination in Feline Immunodeficiency Virus from naturally-infected New Zealand domestic cats Felis catus by sequencing regions of the gag pol and env genes. Results The occurrence of intragenic recombination was highest in env with evidence of recombination in n 156 of all cats. A further recombinant was identified in each of the gag n 48 and pol n 91 genes. Comparisons of phylogenetic trees across genes identified cases of incongruence indicating intergenic recombination. Three n 39 of these incongruencies were found to be significantly different using the Shimodaira-Hasegawa test. Surprisingly our phylogenies from the gag and pol genes showed that no New Zealand sequences group with reference subtype C sequences within intrasubtype pairwise distances. Indeed we find one and two distinct unknown subtype groups in gag and pol respectively. These observations cause us to speculate that these New Zealand

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