tailieunhanh - Báo cáo hóa học: " The product of the Herpes simplex virus 1 UL7 gene interacts with a mitochondrial protein, adenine nucleotide translocator 2"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: The product of the Herpes simplex virus 1 UL7 gene interacts with a mitochondrial protein, adenine nucleotide translocator 2 | Virology Journal BioMed Central Research The product of the Herpes simplex virus I UL7 gene interacts with a mitochondrial protein adenine nucleotide translocator 2 Michiko Tanaka 1 Tetsutaro Sata1 and Yasushi Kawaguchi2 Address Department of Pathology National Institute of Infectious Diseases Shinjuku-ku Tokyo 162-8640 Japan and 2Department of Infectious Disease Control International Research Center for Infectious Disease The Institute of Medical Science The University of Tokyo Tokyo 108-8639 Japan Email Michiko Tanaka - miti@ Tetsutaro Sata - tsata@ Yasushi Kawaguchi - ykawagu@ Corresponding author Open Access Published 22 October 2008 Received 22 August 2008 __iaaao - J- . IA I I ox 11 n - a A- V r n r Accepted 22 October 2008 Virology Journal 2008 5 125 doi 1743-422X-5-125 This article is available from http content 5 1 125 2008 Tanaka et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The herpes simplex virus I HSV-1 UL7 gene is highly conserved among herpesviridae. Since the construction of recombinant HSV-1 with a mutation in the UL7 gene has not been reported the involvement of HSV-1 UL7 in viral replication has been unclear. In this study we succeeded in generating a UL7 null HSV-I mutant virus MTI02 and characterized it. Our results were as follows. i In Vero cells MT102 was replication-competent but formed smaller plaques and yielded 10- to 100-fold fewer progeny than the wild-type virus depending on the multiplicity of infection. ii Using mass spectrometry-based proteomics technology we identified a cellular mitochondrial protein adenine nucleotide translocator 2 ANT2 as a UL7-interacting partner. iii When ANT2 was transiently .