tailieunhanh - Báo cáo hóa học: " Inhibition of G1P3 expression found in the differential display study on respiratory syncytial virus infection"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Inhibition of G1P3 expression found in the differential display study on respiratory syncytial virus infection | Virology Journal BioMed Central Research Inhibition of G1P3 expression found in the differential display study on respiratory syncytial virus infection Dongchi Zhao 1 2 Dan Peng1 Lei Li2 Qiwei Zhang2 and Chuyu Zhang2 Open Access Address Pediatrics Department Zhongnan Hospital of Wuhan University Medical School Donghu Road 169 Wuhan 430071 PR China and 2Virology Institute College of Life Science Wuhan University Wuhan 430072 PR China Email Dongchi Zhao - zhaodong@ Dan Peng - pengdan83@ Lei Li - avlab@ Qiwei Zhang - avlab@ Chuyu Zhang - avlab@ Corresponding author Published 6 October 2008 Received 17 August 2008 Accepted 6 October 2008 Virologyjournal 2008 5 114 doi l743-422X-5-ll4 This article is available from http content 5 l l 14 2008 Zhao et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Respiratory syncytial virus RSV is the leading viral pathogen associated with bronchiolitis and lower respiratory tract disease in infants and young children worldwide. The respiratory epithelium is the primary initiator of pulmonary inflammation in RSV infections which cause significant perturbations of global gene expression controlling multiple cellular processes. In this study differential display reverse transcription polymerase chain reaction amplification was performed to examine mRNA expression in a human alveolar cell line SPC-Al infected with RSV. Results Of the 2 500 interpretable bands on denaturing polyacrylamide gels 40 cDNA bands were differentially regulated by RSV in which 28 70 appeared to be upregulated and another 12 30 appeared to be downregulated. Forty of the expressed sequence tags EST were isolated and 20