tailieunhanh - Báo cáo hóa học: " In vitro effects of selenium deficiency on West Nile virus replication and cytopathogenicity"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: In vitro effects of selenium deficiency on West Nile virus replication and cytopathogenicity | Virology Journal BioMed Central Open Access In vitro effects of selenium deficiency on West Nile virus replication and cytopathogenicity Saguna Verma1 3 Yanira Molina1 3 Yeung Y Lo1 3 Bruce Cropp1 3 Cheynie Nakano1 3 Richard Yanagihara1 2 3 and Vivek R Nerurkar 1 3 Address 1Retrovirology Research Laboratory Department of Tropical Medicine Medical Microbiology and Pharmacology John A. Burns School of Medicine University of Hawaii at Manoa Honolulu HI 96813 USA 2Department of Pediatrics John A. Burns School of Medicine University of Hawaii at Manoa Honolulu HI 96813 USA and 3Asia-Pacific Institute of Tropical Medicine and Infectious Diseases John A. Burns School of Medicine University of Hawaii at Manoa Honolulu HI 96813 USA Email Saguna Verma - saguna@ Yanira Molina - yaniralm@ Yeung Y Lo - Bruce Cropp - cropp@ Cheynie Nakano - cheynie@ Richard Yanagihara - yangiha@ Vivek R Nerurkar - nerurkar@ Corresponding author Published 31 May 2008 Received 25 March 2008 Accepted 3 I May 2008 Virologyjournal 2008 5 66 doi 1743-422X-5-66 This article is available from http content 5 1 66 2008 Verma et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Selenium Se deficiency plays an important role in viral pathogenesis. To understand the effects of Se deficiency on West Nile virus WNV infection we analyzed cytopathogenicity apoptosis and viral replication kinetics using a newly developed Se-deficient cell culture system. Results Both Vero and SK-N-SH cells grown in Se-deficient media exhibited a gradual loss of glutathione peroxidase GPx1 activity without any significant effect on cell growth and .