tailieunhanh - Báo cáo hóa học: " Molecular and macromolecular alterations of recombinant adenoviral vectors do not resolve changes in hepatic drug metabolism during infection"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Molecular and macromolecular alterations of recombinant adenoviral vectors do not resolve changes in hepatic drug metabolism during infection | Virology Journal BioMed Central Open Access Molecular and macromolecular alterations of recombinant adenoviral vectors do not resolve changes in hepatic drug metabolism during infection Shellie M Callahan1 Piyanuch Wonganan1 and Maria A Croyle 1 2 Address 1College of Pharmacy Division of Pharmaceutics The University of Texas at Austin Austin TX USA and institute of Cellular and Molecular Biology The University of Texas at Austin Austin TX USA Email Shellie M Callahan - smcallahan@ Piyanuch Wonganan - piyanuch@ Maria ACroyle - macroyle@ Corresponding author Published 30 September 2008 Virology Journal 2008 5 111 doi l743-422X-5-ll I Received 8 August 2008 Accepted 30 September 2008 This article is available from http content 5 l l ll 2008 Callahan et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract In this report we test the hypothesis that long-term virus-induced alterations in CYP occur from changes initiated by the virus that may not be related to the immune response. Enzyme activity protein expression and mRNA of CYP3A2 a correlate of human CYP3A4 and CYP2Cll responsive to inflammatory mediators were assessed l 4 and l4 days after administration of several different recombinant adenoviruses at a dose of X l0l2 virus particles vp kg to male Sprague Dawley rats. Wild type adenovirus containing all viral genes suppressed CYP3A2 and 2Cll activity by 37 and 39 respectively within six hours. Levels fell to 67 CYP3A2 and 79 CYP2Cl l of control by l4 days p . Helper-dependent adenovirus with all viral genes removed suppressed CYP3A2 43 and CYP2Cl l 55 within six hours. CYP3A2 remained significantly suppressed 47 l4 days p while CYP2Cll