tailieunhanh - Báo cáo hóa học: " Transient expression of homologous hairpin RNA interferes with PVY transmission by aphids"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Transient expression of homologous hairpin RNA interferes with PVY transmission by aphids | Virology Journal BioMed Central Open Access Short report Transient expression of homologous hairpin RNA interferes with PVY transmission by aphids Marisol Vargas Belén Martinez-Garcia José Ramón Díaz-Ruíz and Francisco Tenllado Address Departamento de Biología de Plantas Centro de Investigaciones Biológicas CIB CSIC Campus de la Ciudad Universitaria Av. Ramiro de Maeztu 9 28040 Madrid Spain Email Marisol Vargas - marisolvargasco@ Belén Martínez-García - belenmaga@ José Ramón Díaz- Ruíz - jrdiazruiz@ Francisco Tenllado - tenllado@ Corresponding author Published 19 March 2008 Received 29 January 2008 Accepted 19 March 2008 Virologyjournal 2008 5 42 doi l743-422X-5-42 This article is available from http content 5 1 42 2008 Vargas et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Hairpin RNAs have been used to confer resistance to viruses in plants through RNA silencing. However it has not been demonstrated that RNA silencing was effective against inoculation by aphids of non-persistently transmitted viruses the major route of plant virus spread in nature. As a proof-of-principle strategy we made use of Agrobacterium tumefaciens to transiently express a hairpin RNA homologous to Potato virus Y PVY in plant tissues. A complete and specific interference with aphid transmission of PVY was achieved by inducers of RNA silencing as demonstrated by specific siRNAs accumulation in agroinfiltrated tissues. To our knowledge this is the first report of successful interference with non-persistent transmission of a plant virus using RNA interference. Findings One of the most efficient mechanisms by which plants protect themselves from viruses is the specific .