tailieunhanh - Báo cáo hóa học: " Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug "

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug | Virology Journal BioMed Central Research Vaccinia virus lacking the deoxyuridine triphosphatase gene F2L replicates well in vitro and in vivo but is hypersensitive to the antiviral drug N -methanocarbathymidine Mark N Prichard 1 Earl R Kern1 Debra C Quenelle1 Kathy A Keith1 Richard W Moyer2 and Peter C Turner2 Address Department of Pediatrics University of Alabama School of Medicine Birmingham AL 35233 USA and - Department of Molecular Genetics and Microbiology University of Florida College of Medicine Gainesville FL 32610 USA Email Mark N Prichard - mprichard@ Earl R Kern - ekern@ Debra C Quenelle - dquenelle@ Kathy A Keith - kkeith@ Richard W Moyer - rmoyer@ Peter C Turner - pturner@ Corresponding author Open Access Published 5 March 2008 Received 24 January 2008 Accepted 5 March 2008 Virology journal 2008 5 39 doi 1743-422X-5-39 This article is available from http content 5 1 39 2008 Prichard et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The vaccinia virus VV F2L gene encodes a functional deoxyuridine triphosphatase dUTPase that catalyzes the conversion of dUTP to dUMP and is thought to minimize the incorporation of deoxyuridine residues into the viral genome. Previous studies with with a complex multigene deletion in this virus suggested that the gene was not required for viral replication but the impact of deleting this gene alone has not been determined in vitro or in vivo. Although the crystal structure for this enzyme has been determined its potential as a target for antiviral therapy is unclear. Results The F2L gene was replaced with GFP in the WR strain of VV to assess its effect on viral .