tailieunhanh - Báo cáo hóa học: " Frag-Virus: a new term to distinguish presumptive viruses known primarily from sequence data"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Frag-Virus: a new term to distinguish presumptive viruses known primarily from sequence data | Virology Journal BioMed Central Open Access Debate Frag-Virus a new term to distinguish presumptive viruses known primarily from sequence data Alexander Voevodin 1 and Preston A Marx2 Address 1Vir Gen Toronto Canada and 2Division of Microbiology Tulane National Primate Research Center Covington Louisiana USA Email Alexander Voevodin - voevodin@ Preston A Marx - pmarx@ Corresponding author Published 27 February 2008 Received 18 February 2008 Accepted 27 February 2008 Virology journal 2008 5 34 doi l743-422X-5-34 This article is available from http content 5 1 34 2008 Voevodin and Marx licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. The wide availability of PCR-based methods for the amplification of nucleic acids homologous to known viral genomes plus automated DNA sequencing have led to explosive growth of a new field proxy virus isolation. Typically genomic fragments are amplified and subjected to phylogenetic analysis showing that they are related to but distinct from known bona fide viruses. These new viruses are now commonly reported in the virological literature and are too numerous to cite here two studies are given as typical examples 1 2 . Although unintentional these reports may mislead the readership of scientific journals and the general press. Having no distinction between preliminary genome-based evidence and conclusive proof by biological isolation and characterization of a replication-competent virus blurs the meaning of new virus. To distinguish presumptive viruses known primarily through genomic sequence fragments from bona fide viruses we propose the term frag-virus . The frag-virus status may be intermediary between full recognition as a new virus as occurred