tailieunhanh - Báo cáo hóa học: " Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses | Virology Journal BioMed Central Open Access Methodology Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses Parag Saxena Paban Kumar Dash SR Santhosh Ambuj Shrivastava Manmohan Parida and PV Lakshmana Rao Address Division of Virology Defence Research Development Establishment Jhansi Road Gwalior 474 002 MP India Email Parag Saxena - paragsaxena_viro@ Paban Kumar Dash - pabandash@ SR Santhosh - santhuvet4u@ Ambuj Shrivastava - ambujshrivastava@ Manmohan Parida - paridamm@ PV Lakshmana Rao - pvlrao@ Corresponding author Published 30 January 2008 Received 27 November 2007 Accepted 30 January 2008 Virology Journal 2008 5 20 doi l 743-422X-5-20 This article is available from http content 5 1 20 2008 Saxena et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Dengue is emerging as a major public health concern in many parts of the world. The development of a one-step single tube rapid and multiplex reverse transcription polymerase chain reaction M-RT-PCR for simultaneous detection and typing of dengue virus using serotype specific primers during acute phase of illness is reported. Results An optimal assay condition with zero background was established having no crossreaction with closely related members of flavivirus Japanese encephalitis West Nile Yellow fever and alphavirus Chikungunya . The feasibility of M-RT-PCR assay for clinical diagnosis was validated with 620 acute phase dengue patient sera samples of recent epidemics in India. The comparative .