tailieunhanh - Báo cáo hóa học: " Self-excision of the BAC sequences from the recombinant Marek's disease virus genome increases replication and pathogenicity"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Self-excision of the BAC sequences from the recombinant Marek's disease virus genome increases replication and pathogenicity | Virology Journal BioMed Central Short report Open Access Self-excision of the BAC sequences from the recombinant Marek s disease virus genome increases replication and pathogenicity Yuguang Zhao Lawrence Petherbridge Lorraine P Smith Sue Baigent and Venugopal Nair Address Viral Oncogenesis Group Institute for Animal Health Compton Berkshire RG20 7NN UK Email YuguangZhao - Lawrence Petherbridge - Lorraine P Smith - Sue Baigent - Venugopal Nair - Corresponding author Published 30 January 2008 Received 12 July 2007 Accepted 30 January 2008 Virology Journal 2008 5 19 doi 1743-422X-5-19 This article is available from http content 5 1 19 2008 Zhao et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Cloning of full length genomes of herpesviruses as bacterial artificial chromosomes BAC has greatly facilitated the manipulation of the genomes of several herpesviruses to identify the pathogenic determinants. We have previously reported the construction of the BAC clone pRB-1B5 of the highly oncogenic Marek s disease virus MDV strain RB-1B which has proven to be a valuable resource for elucidating several oncogenic determinants. Despite the retention of the BAC replicon within the genome the reconstituted virus was able to induce tumours in susceptible chickens. Nevertheless it was unclear whether the presence of the BAC influenced the full oncogenic potential of the reconstituted virus. To maximize the closeness of BAC-derived virus to the parental RB-1B strain we modified the existing pRB-1B5 clone by restoring the Us2 and by introducing SV40-cre cassette within .