tailieunhanh - báo cáo khoa học: " Antitumor activity of mixed heat shock protein/ peptide vaccine and cyclophosphamide plus interleukin-12 in mice sarcoma"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Antitumor activity of mixed heat shock protein/ peptide vaccine and cyclophosphamide plus interleukin-12 in mice sarcoma | Guo et al. Journal of Experimental Clinical Cancer Research 2011 30 24 http content 30 1 24 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Antitumor activity of mixed heat shock protein peptide vaccine and cyclophosphamide plus interleukin-12 in mice sarcoma Quan-Yi Guo Mei Yuan Jiang Peng Xue-Mei Cui Ge Song Xiang Sui Shi-Bi Lu Abstract Background The immune factors heat shock protein HSP peptides HSP Ps can induce both adaptive and innate immune responses. Treatment with HSP Ps in cancer cell-bearing mice and cancer patients revealed antitumor immune activity. We aimed to develop immunotherapy strategies by vaccination with a mixture of HSP Ps mHSP Ps HSP60 HSP70 Gp96 and HSP110 enhanced with cyclophosphamide CY and interleukin-12 IL-12 . Methods We extracted mHSP Ps from the mouse sarcoma cell line S180 using chromatography. The identity of proteins in this mHSP Ps was assayed using SDS-PAGE and Western blot analysis with antibodies specific to various HSPs. BALB C mice bearing S180 cells were vaccinated with mHSP Ps x3 then were injected intraperitoneally with low-dose CY and subcutaneously with IL-12 100 pg day x5. After vaccination T lymphocytes in the peripheral blood were analyzed using FACScan and Cytotoxicity CTL was analyzed using lactate dehydrogenase assay. ELISPOT assay was used to evaluate interferon y IFN-y and immune cell infiltration in tumors was examined in the sections of tumor specimen. Results In mice vaccinated with enhanced vaccine mHSP Ps and CY plus IL-12 80 showed tumor regression and long-term survival and tumor growth inhibition rate was 30 days all controls died within 40 days. After vaccination lymphocytes and polymorphonuclear leukocytes infiltrated into the tumors of treated animals but no leukocytes infiltrated into the tumors of control mice. The proportions of natural killer cells CD8 and interferon-y-secreting cells were all increased in the immune group and tumor-specific cytotoxic T .