tailieunhanh - báo cáo khoa học: " LgR5 expression and cancer stem cell hypothesis: clue to define the true origin of esophageal adenocarcinomas with and without Barrett’s Esophagus?"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: LgR5 expression and cancer stem cell hypothesis: clue to define the true origin of esophageal adenocarcinomas with and without Barrett’s Esophagus? | von Rahden et al. Journal of Experimental Clinical Cancer Research 2011 30 23 http content 30 1 23 Journal of Experimental Clinical Cancer Research RESEARCH Open Access LgR5 expression and cancer stem cell hypothesis clue to define the true origin of esophageal adenocarcinomas with and without Barrett s Esophagus 1 2 3 1 1 1 Burkhard HA von Rahden Stefan Kircher Maria Lazariotou Christoph Reiber Luisa Stuermer Christoph Otto Christoph T Germer1 Martin Grimm1 Abstract Background Investigation of the expression of an intestinal stem cell marker in esophageal adenocarcinomas EAC with and without Barrett s Esophagus BE with respect to a cancer stem cell CSC hypothesis. Materials and methods Expression of a putative intestinal stem cell marker LgR5 was analyzed in esophageal cancer specimen n 70 41 EAC with BE 19 EAC without BE and n 10 esophageal squamous-cell carcinomas ESCC and in the adenocarcinoma cell line OE-33. Ki-67 and Cdx-2 were co-labelled with LgR5 in double staining experiments. Immunhistochemical expression results were confirmed by RT-PCR and correlated with tumor stage and five-year survival rates. Results LgR5was found expressed in 35 of 41 85 EAC with BE and in 16 of 19 81 EAC without BE. By contrast LgR5 was not found to be expressed in ESCC. Quantification of immunolabeling showed 15 LgR5 cells in EAC with BE 32 LgR5 cells in adjacent BE and 13 in EAC without BE. Immunofluorescence double staining experiments with LgR5 and Ki-67 revealed a subpopulation 5 of proliferating LgR Ki-67 cells. On mRNA-level expression of LgR5 was higher in BE in comparison to EAC p . High levels of LgR5 expression in BE associated EAC were associated with poorer survival in univariate analysis. Conclusion The stem cell marker LgR5 is expressed in EAC irrespective of association with BE and appears to have negative impact on survival. The subset of proliferating LgR5 cells 5 might resemble rapidly cycling CSCs which needs to be substantiated in .

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