tailieunhanh - báo cáo khoa học: "Leptin promotes melanoma tumor growth in mice related to increasing circulating endothelial progenitor cells numbers and plasma NO production"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Leptin promotes melanoma tumor growth in mice related to increasing circulating endothelial progenitor cells numbers and plasma NO production | Amjadi et al. Journal of Experimental Clinical Cancer Research 2011 30 21 http content 30 1 21 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Leptin promotes melanoma tumor growth in mice related to increasing circulating endothelial progenitor cells numbers and plasma NO production 1 1 2 3 Fatemehsadat Amjadi Shaghaygh Haghjooy Javanmard Hamid Zarkesh-Esfahani Majid Khazaei Manijeh Narimani1 Abstract Background Epidemiological studies propose that obesity increases the risk of several cancers including melanoma. Obesity increases the expression of leptin a multifunctional peptide produced predominantly by adipocytes which may promote tumor growth. Several recently experiments have suggested that the tumors growth is in need of endothelial progenitor cell EPC dependent generation of new blood vessels. Our objectives in the present study were to examine the effects of leptin on melanoma growth circulating EPCs number and plasma levels of nitric oxide metabolites NOx . Methods 2 X 106 B16F10 melanoma cells were injected to thirty two C57BL6 mice subcutaneously. The mice were randomly divided into 4 groups n 8 in 8th day. Two groups were received twice daily intraperitoneal injections of either PBS or recombinant murine leptin 1 gg g initial body weight . Two groups were received . injections of either 9F8 an anti leptin receptor antibody or the control mouse IgG at 50 gg mouse every 3 consecutive days. By the end of the second week the animals were euthanized and blood samples and tumors were analyzed. Results The tumor weight EPC numbers and NOx level in leptin PBS 9F8 and IgG group were g 171 18 ml of blood and gmol L respectively. Tumors weight and size circulating EPC numbers and plasma levels of NOx were significantly more in the leptin than 9f8 and both control groups p . The plasma concentration of NOx