tailieunhanh - báo cáo khoa học: " The function and mechanism of COX-2 in angiogenesis of gastric cancer cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The function and mechanism of COX-2 in angiogenesis of gastric cancer cells | Journal of Experimental Clinical Cancer Research Yao et al. Journal of Experimental Clinical Cancer Research 2011 30 13 http content 30 1 13 RESEARCH Open Access The function and mechanism of COX-2 in angiogenesis of gastric cancer cells Liping Yaot Fei Liu Liu Hong1 Li Sun Shuhui Liang Kaichun Wu Daiming Fan Abstract Background Here we aimed to investigate the effect of COX-2 siRNA on proliferation and angiogenesis of gastric cancer cells. Methods The gastric cancer cell line SGC7901 was transfected with COX-2 siRNA then the growth and angiogenesis of cells were detected by in vitro and in vivo assay. Human microarray RT-PCR and western blot were used to identify differentially expressed angiogenesis-related molecules in cells with decreased expression of COX-2. Results Down-regulation of COX-2 could significantly inhibit the in vitro and in vivo growth of gastric cancer cells and suppress the migration and tube formation of human umbilical vein endothelial cells. Totally 23 angiogenesis-related molecules were found involved in COX-2-induced angiogenesis suppression. The results of RT-PCR and western blot showed that down-regulation of COX-2 might inhibit VEGF Flt-1 Flk-1 KDR angiopoietin-1 tie-2 MMP2 and OPN. Conclusions COX-2 might mediate tumor angiogenesis and growth and could be considered as a target for gastric cancer therapy. Background Gastric cancer is the second leading cause of cancer associated death in the world particularly in Asian countries. The treatment outcome of this common malignancy is still not satisfactory and various chemotherapeutic attempts in an adjuvant setting have failed to improve the survival rate in gastric cancer. Recently angiogenesis has been found related to hematogenous recurrence and poor prognosis in gastric cancer 1 . Angiogenesis is the growth of new vessels from existing vasculature. A balance of angiogenic and angiostatic growth factors tightly controls physiological angiogenesis. Tipping of this balance