tailieunhanh - báo cáo khoa học: " Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells | Zhang et al. Journal of Experimental Clinical Cancer Research 2011 30 98 http content 30 1 98 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells Yi Zhang1 2t Fu-qiang ChenH Ye-hong SunH Shu-yan ZhouH Ti-yuan Li1 and Rui Chen1 2t Abstract Background DNA methylation has been widely used in classification early diagnosis therapy and prediction of metastasis as well as recurrence of cervical cancer. DNMT methyltransferase 1 DNMT1 which plays a significant role in maintaining DNA methylation status and regulating the expression of tumor suppressor genes. The aim of this research was to investigate the relationship between DNMT1 and abnormal methylation of tumor suppressor genes and malignant phenotype in cervical cancer. Methods Levels of DNMT1 mRNA and protein were detected using qPCR and Western blot respectively. Cell proliferation was analyzed by MTT and apoptosis was performed by Annexin V-FITC PI double staining flow cytometry respectively. MeDIP-qPCR and qPCR were performed to measure demethylation status and mRNA reexpression level of 7 tumor-suppressor genes CCNA1 CHFR FHIT PAX1 PTEN SFRP4 TSLC1 in Hela and Siha cells after silencing DNMT1. Results The average expression levels of DNMT1 mRNA and protein in Hela and Siha cells were decreased significantly compared with control group. The flow cytometry and MTT results showed that Hela and Siha cells apoptosis rates and cell viabilities were as well as respectively 48 h after transfection P . Furthermore the promoter methylation of five tumor suppressor genes was decreased with the increased mRNA expression after silencing DNMT1 whereas there were no significant changes in PTEN and FHIT genes in Hela cells and CHFR and FHIT genes in Siha cells. Conclusions Our experimental results demonstrate that methylation status of DNMT1 can .