tailieunhanh - Báo cáo hóa học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome"

Tham khảo tài liệu 'báo cáo hóa học: " cellular apoptosis induced by replication of hepatitis b virus: possible link between viral genotype and clinical outcome"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Virology Journal BioMed Central Short report Open Access Cellular apoptosis induced by replication of hepatitis B virus possible link between viral genotype and clinical outcome Yi Wei Lu Tuan Lin Tan Jianhua Zhang and Wei Ning Chen Address School of Chemical and Biomedical Engineering Nanyang Technological University 62 Nanyang Drive 637459 Singapore Email Yi Wei Lu - YWLu@ Tuan Lin Tan - TLTan@ Jianhua Zhang - JHZhang@ Wei Ning Chen - wnchen@ Corresponding author Published 31 October 2007 Virology Journal 2007 4 117 doi 1743-422X-4-11 7 Received 5 September 2007 Accepted 3l October 2007 This article is available from http content 4 l l 17 2007 Lu et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract HBV remains one of the major pathogens of liver diseases but the outcomes as inflammation cirrhosis and cancer of the liver are greatly related to different viral genotypes. The aim of this study was to assess the pro-apoptotic effect of HBSP from three HBV genotypes on liver derived cells. HepG2 cells were applied in our system and transfected by HBV genotype A B and C. Cells were observed under phase contrast microscope stained by apoptosis marker and analyzed by flow cytometre. HBSP expression was detected by western blot assay. BH3 sequences were aligned and analyzed by Vector NTI. HBV genotypes A B and C transfected cells displayed evidence of cell death which was further proved as apoptosis. Natural expression of a pro-apoptotic protein HBSP was detected during genomes transfection. The different apoptotic effects were correlated to the HBSP expression from each genome. Alignment and analysis of the BH3 domains from the three genomes revealed .