tailieunhanh - báo cáo khoa học: "The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma | Huang et al. Journal of Experimental Clinical Cancer Research 2011 30 88 http content 30 1 88 Journal of Experimental Clinical Cancer Research RESEARCH Open Access The expression and role of protein kinase C PKC epsilon in clear cell renal cell carcinoma o IL . I_I I I 1 t I - I i- r. s f 2 t V 11 1r I 12 c h I If I I z- 4 V1 r-v 1 k I s f 1 7 h II A I z i2 I I f ĩ hl I I z t 1 Din Huang Kaiyuan Cao Ăiubo LI Shengjie Guo Ăiaopeng Mao Zhu Wang Jintao Zhuang Jincheng Pan1 Chengqiang Mo1 Junxing Chen1 and Shaopeng Qiu1 Abstract Protein kinase C epsilon PKCe an oncogene overexpressed in several human cancers is involved in cell proliferation migration invasion and survival. However its roles in clear cell renal cell carcinoma RCC are unclear. This study aimed to investigate the functions of PKCe in RCC especially in clear cell RCC to determine the possibility of using it as a therapeutic target. By immunohistochemistry we found that the expression of PKCe was up-regulated in RCCs and was associated with tumor Fuhrman grade and T stage in clear cell RCCs. Clone formation wound healing and Borden assays showed that down-regulating PKCs by RNA interference resulted in inhibition of the growth migration and invasion of clear cell RCC cell line 769P and more importantly sensitized cells to chemotherapeutic drugs as indicated by enhanced activity of caspase-3 in PKCs siRNA-transfected cells. These results indicate that the overexpression of PKCs is associated with an aggressive phenotype of clear cell RCC and may be a potential therapeutic target for this disease. Keywords Protein kinase C epsilon Renal cell carcinoma Clear cell Background Renal cell carcinoma RCC accounts for approximately 3 of all malignant tumors in adults which afflicts about 58 240 people and causes nearly 13 040 deaths each year in USA 1 . RCCs are classified into five major subtypes clear cell the most important type accounts for 82 papillary chromophobe collecting duct and unclassified

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