tailieunhanh - báo cáo khoa học: " Aquaporin-3 positively regulates matrix metalloproteinases via PI3K/AKT signal pathway in human gastric carcinoma SGC7901 cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Aquaporin-3 positively regulates matrix metalloproteinases via PI3K/AKT signal pathway in human gastric carcinoma SGC7901 cells | Xu et al. Journal of Experimental Clinical Cancer Research 2011 30 86 http content 30 1 86 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Aquaporin-3 positively regulates matrix metalloproteinases via PI3K AKT signal pathway in human gastric carcinoma SGC7901 cells Hao Xu Yong Xu Wenjie Zhang Lizong Shen Li Yang and Zekuan Xu Abstract Background matrix metalloproteinases MMPs are produced by tumor cells so they may be associated with tumor progression including invasion migration angiogenesis and metastasis. Aquaporin-3 AQP3 also plays a critical role in gastric cancer cell migration and proliferation. Methods In this study AQP3 was silenced or over-expressed in SGC7901 cells. Results We found a significant decrease in MT1-MMP MMP-2 and MMP-9 expression after AQP3 knockdown and a significant increase in MT1-MMP MMP-2 and MMP-9 expression after AQP3 over-expression in SGC7901 cells. We also found that AQP3 silence led to a significant decrease of phosphorylation of ser473 in AKT in SGC7901 cells. Conclusion Our findings showed that AQP3 might positively regulate MMPs proteins expression through PI3K AKT signal pathway in human gastric carcinoma SGC7901 cells. Keywords Aquaporin-3 Matrix metalloproteinase PI3K AKT pathway Background Gastric cancer GC remains a major cause of mortality and morbidity worldwide 1 . The rapid invasion and metastasis of tumor cells are responsible for poor prognosis 2 . The high expression of MMPs in GC tissues has been determined in several studies 3 4 . It has shown that MMPs expression correlates with clinical pathological features of GC such as tumor stage depth of tumor invasion and the presence of lymph node and distant metastases 5 . Aquaporins AQPs are a family of small 30 kDa monomer hydrophobic integral membrane proteins which belong to a special superfamily of membrane integral proteins called MIPs major intrinsic proteins 6 7 . In our previous work we showed a differential expression of .