tailieunhanh - Báo cáo hóa học: " Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein"

Tham khảo tài liệu 'báo cáo hóa học: " nucleocapsid formation and rna synthesis of marburg virus is dependent on two coiled coil motifs in the nucleoprotein"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Virology Journal BioMed Central Research Open Access Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein Andrea DiCarlo1 2 Peggy Moller1 3 Angelika Lander1 4 Larissa Kolesnikova1 4 and Stephan Becker 1 4 Address 1Philipps-Universitat Marburg Institut fur Virologie Hans Meerwein-Str. 2 35032 Marburg Germany 2Promega GmbH High-TechPark Schildkrotstrafie 15 D-68199 Mannheim Germany 3Paul Ehrlich-Institut Paul-Ehrlich-Str. 51 - 59 63225 Langen Germany and 4Robert Koch-Institut Zentrum fur Biologische Sicherheit Berlin Nordufer 20 13353 Berlin Germany Email Andrea DiCarlo - Peggy Moller - moepe@ Angelika Lander - LanderA@ Larissa Kolesnikova - KolesnikovaL@ Stephan Becker - BeckerSt@ Corresponding author Published 24 October 2007 Received II September 2007 Accepted 24 October 2007 Virology Journal 2007 4 105 doi 1743-422X-4-105 This article is available from http content 4 1 105 2007 DiCarlo et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The nucleoprotein NP of Marburg virus MARV is responsible for the encapsidation of viral genomic RNA and the formation of the helical nucleocapsid precursors that accumulate in intracellular inclusions in infected cells. To form the large helical MARV nucleocapsid NP needs to interact with itself and the viral proteins VP30 VP35 and L which are also part of the MARV nucleocapsid. In the present study a conserved coiled coil motif in the central part of MARV NP was shown to be an important element for the interactions of NP with itself and VP35 the viral polymerase cofactor. Additionally the coiled coil motif was essential for the formation