tailieunhanh - báo cáo khoa học: " HIF-1a effects on angiogenic potential in human small cell lung carcinoma"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: HIF-1a effects on angiogenic potential in human small cell lung carcinoma | Journal of Experimental Clinical Cancer Research Wan et al. Journal of Experimental Clinical Cancer Research 2011 30 77 http content 30 1 77 RESEARCH Open Access HIF-1a effects on angiogenic potential in human small cell lung carcinoma Jun Wan Huiping Chap Zaicheng Yu Wei Ge Ningning Kang Wanli Xia and Yun Che Abstract Background Hypoxia-inducible factor-1 alpha HIF-1a maybe an important regulatory factor for angiogenesis of small cell lung cancer SCLC . Our study aimed to investigate the effect of HIF-1a on angiogenic potential of SCLC including two points One is the effect of HIF-1a on the angiogenesis of SCLC in vivo. The other is the regulation of angiogenic genes by HIF-1a in vitro and in vivo. Methods In vivo we used an alternative method to study the effect of HIF-1a on angiogenic potential of SCLC by buliding NCI-H446 cell transplantation tumor on the chick embryo chorioallantoic membrane CAM surface. In vitro we used microarray to screen out the angiogenic genes regulated by HIF-1a and tested their expression level in CAM transplantation tumor by RT-PCR and Western-blot analysis. Results In vivo angiogenic response surrounding the SCLC transplantation tumors in chick embryo chorioallantoic membrane CAM was promoted after exogenous HIF-1a transduction p . In vitro the changes of angiogenic genes expression induced by HIF-1a in NCI-H446 cells were analyzed by cDNA microarray experiments. HIF-1a upregulated the expression of angiogenic genes VEGF-A TNFAIP6 PDGFC FN1 MMP28 MMP14 to fold respectively and glycolytic genes GLUT1 GLUT2 fold respectively. In addition the expression of these angiogenic factors were also upregulated by HIF-1a in the transplantion tumors in CAM as RT-PCR and Western-blot analysis indicated. Conclusions These results indicated that HIF-1a may enhance the angiogenic potential of SCLC by regulating some angiogenic genes such as VEGF-A MMP28 etc. Therefore HIF-1a may be