tailieunhanh - Báo cáo khoa học: "An efficient micro-method of DNA isolation from mature leaves of four hardwood tree species Acer, Fraxinus, Prunus and Quercus"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: An efficient micro-method of DNA isolation from mature leaves of four hardwood tree species Acer, Fraxinus, Prunus and Quercus. | 259 Ann. For. Sci. 56 1999 259-263 Inra Elsevier Paris Note An efficient micro-method of DNA isolation from mature leaves of four hardwood tree species Acer Fraxinus Prunus and Quercus Frangois Lefort a b Gerard c. Douglas3 Teagasc Food and Agriculture Development Authority Kinsealy Research Centre Malahide Road Dublin 17 Ireland b Laboratory of Plant Physiology and Biotechnology Department of Biology University of Crete . Box 208 71409 Heraklio Greece Received 5 January 1998 accepted 1 October 1998 Abstract - It is difficult to purify DNA from mature tree leaves at the end of the growing season because of their thick cell wall and high content in polysaccharides phenolic compounds and endonucleases. A simple fast and efficient method for DNA purification from 100 mg fresh weight leaf samples is described here. It has been developed for extracting DNA from mature leaves of Quercus Fraxinus Primus and Acer. The protocol is a modified CT AB hexadecyltrimethylammonium bromide method including a combination of P-mercaptoethanol polyvinylpyrrolidone sodium dodecyl sulfate and lithium chloride including short centrifugation runs. It is very efficient yielding up to 950 pg DNA g of fresh weight even when very mature leaves are processed. The extracted DNA was used as template to characterise oaks by microsatellite analysis. Its efficiency has been compared to four commercially available kits and two other published. CTAB protocols. The protocol is also inexpensive compared to commercial kits. Inra Elsevier Paris. Acer IDNA purification Fraxinus ì Prunus I Quercus Resume - Une micro-methode d extraction d ADN à partir de feuilles matures de quatre espèces d arbres forestiers Acer Fraxinus Prunus et Quercus. Il est difficile de purifier 1 ADN de feuilles d arbres à maturité et spécialement à la fin de la période de croissance c est-a-dire en automne pour plusieurs raisons telles que de fortes concentrations de polysaccharides de composes phénoliques et d endonucleases .

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