tailieunhanh - Báo cáo hóa học: " Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method"

Tham khảo tài liệu 'báo cáo hóa học: " cloning of full genome sequence of hepatitis e virus of shanghai swine isolate using race method"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Virology Journal BioMed Central Research Open Access Cloning of full genome sequence of hepatitis E virus of Shanghai swine isolate using RACE method Quan Shen1 2 Wen Zhang1 Xiangrong Cao2 Jing Mou1 Li Cui1 and Xiuguo Hua 1 Address 1School of Agriculture and Biology Shanghai JiaoTong University 800 Dongchuan Road Shanghai 200240 PR China and 2School of Life Science Nanjing Normal University 1 Wenyuan Road Nanjing 210097 PR China Email Quan Shen - njnushenquan@ Wen Zhang - z0216wen@ Xiangrong Cao - caoxiangrong@ Jing Mou - shenquanfly@ Li Cui - lcui@ Xiuguo Hua - hxg@ Corresponding author Published 9 October 2007 Received 2 August 2007 Accepted 9 October 2007 Virology Journal 2007 4 98 doi l 743-422X-4-98 This article is available from http content 4 l 98 2007 Shen et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Genotype 4 hepatitis E virus HEV was reportedly transmitted freely between humans and swine in eastern China. The full-length genomic sequence of Shanghai swine isolate SH-SW-zsl recovered from feces sample of a pig which was infected with HEV RNA positive swine serum was determined using RT-PCR and RACE Rapid Amplification of cDNA Ends methods. The full genome of the SH-SW-zsl isolate was 7265 nucleotides in length and phylogenetic analysis indicated that this isolate belonged to genotype 4. Comparison of the 3 UTR sequence with the corresponding regions of other 38 HEV strains from different region revealed that the Shanghai swine isolate is 2l-49 bp longer than the other stains. Introduction Hepatitis E is an important public health disease in many developing countries of Asia and Africa and also occurs .