tailieunhanh - báo cáo khoa học: "Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK | Luo et al. Journal of Experimental Clinical Cancer Research 2011 30 71 http content 30 1 71 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA uPAR and ERK Jie Luo Xin Sun Feng Gao Xiaoliang Zhao Biao Zhong Hong Wang and Zhijun Sun Abstract Objective To investigate the effects of ulinastatin and docetaxel on invasion of breast cancer cells and expression of uPA uPAR and ERK breast cancer MDA-MB-231 and MCF-7 cells. Methods The nude mice were treated with PBS ulinastatin docetaxel and ulinastatin plus docetaxel respectively. Their effects on 1 cell invasion ability was assayed using Transwell 2 expression of uPA uPAR and ERK was detected by real time PCR and Western blot 3 uPA uPAR and p-ERK protein level in nude mice was quantified by immunohistochemistry. Results 1 Treatment with ulinastatin docetaxel and ulinastatin plus docetaxel respectively significantly inhibited MDA-MB-231 and MCF-7 cell invasion 2 mRNA and protein levels of uPA uPAR and ERK1 2 were inhibited by ulinastatin but enhanced by docetaxel. Conclusion Ulinastatin can enhance the effects of docetaxel on invasion of breast cancer cells. And that uPA uPAR and p-ERK expression is obviously inhibited by ulinastatin. Introduction Breast cancer is one of the major malignant tumors threaten women well being. Failure in its treatment mainly arises from cancer proliferation invasion and metastasis which ultimately lead to the death of patients. Cell penetrating into extracellular base membrane is the premise of cancer cell metastasis where a variety of proteases play essential roles. Plasminogen activators PAs are serine proteases the main function of which is to activate plasminogen into plasmin a serine protease that hydrolyzes a variety of proteins including laminin fibronectin fibrin proteoglycan core protein and collagen fibres. There are two types of mammalian PAs tissue-type tPA and .