tailieunhanh - báo cáo khoa học: " Multi-drug resistance 1 genetic polymorphism and prediction of chemotherapy response in Hodgkin’s "

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Multi-drug resistance 1 genetic polymorphism and prediction of chemotherapy response in Hodgkin’s | Mhaidat et al. Journal of Experimental Clinical Cancer Research 2011 30 68 http content 30 1 68 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Multi-drug resistance 1 genetic polymorphism and prediction of chemotherapy response in Hodgkin s Lymphoma 1 1 2 1 3 Nizar M Mhaidat Osama Y Alshogran Omar F Khabour Karem H Alzoubi Ismail I Matalka William J Haddadin4 Ibraheem O Mahasneh5 and Ahmad N Aldaher 1 Abstract Background The human multi-drug resistance gene MDR1 which encodes the major trans-membrane transporter P-glycoprotein P-gp was found to be associated with susceptibility to cancer and response to chemotherapy. The C3435T Polymorphism of MDR1 gene was correlated with expression levels and functions of P-gp. Here we studied the association between MDR1 C3435T polymorphism and susceptibility to Hodgkin lymphoma HL and patient s response to ABVD chemotherapy regimen. Methods a total of 130 paraffin embedded tissue samples collected from HL patients were analyzed to identify the C3435T polymorphism. As a control group 120 healthy subjects were enrolled in the study. The C3435T Polymorphism was genotyped by polymerase chain reaction and restriction fragment length polymorphism PCR-RFLP method. Data analysis was carried out using the statistical package SPSS version 17 to compute all descriptive statistics. Chi-square and Fisher exact tests were used to evaluate the genotype distribution and allele frequencies of the studied polymorphism. Results these studies revealed that the frequency of T allele was significantly higher in HL patients compared to the controls P . In addition the frequency of CT and TT genotypes were also significantly higher in HL patients compared to the controls P . No association between C3435T polymorphism and response to ABVD was detected among HL patients P . Conclusions these results suggest that MDR1 C3435T polymorphism might play a role in HL occurrence however this polymorphism is .