tailieunhanh - báo cáo khoa học: " XAF1 expression and regulatory effects of somatostatin on XAF1 in prostate cancer cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: XAF1 expression and regulatory effects of somatostatin on XAF1 in prostate cancer cells | Xing et al. Journal of Experimental Clinical Cancer Research 2010 29 162 http content 29 1 162 RESEARCH ÍOI It I Journal of Experimental Clinical Cancer Research Open Access XAF1 expression and regulatory effects of somatostatin on XAF1 in prostate cancer cells 7hcvc11 Ian y i I-IC 111 7 m I i I-I 7he ll I2t -Zwi Vl I3 4 chi ilii-ie 1 I i 1Ch liedd I 4 toe Milcce in4 XL .lew I I II I2 3 Zhaoquan Aing zuiilin Zhou Rong iu Shuling LI cnunue LI Seen Nilsson Zhaoxu Liu Abstract Background Somatostatin prevents cell proliferation by Inducing apoptosis. Downregulation of the XAF1 transcript may occur during the development of prostate cancer. It is interesting to evaluate the potential regulatory effects of somatostatin on XAF1 expression during the development of prostate cancer cells. Methods XAF1 mRNA and protein expression in human prostate epithelial cells RWPE-1 androgen dependent prostate cancer LNCaP and androgen independent DU145 and PC3 cells were evaluated using RT-PCR and Western blot. The regulation of XAF1 mRNA and protein expression by somatostatin and its analogue Octreotide was evaluated. Results Substantial levels of XAF1 mRNA and proteins were detected in RWPE-1 cells whereas prostate cancer cells LNCaP DU145 and PC3 exhibited lower XAF1 expression. Somatostatin and Octreotide up-regulated XAF1 mRNA and protein expression in all prostate cancer cell lines. Conclusions XAF1 down-regulation may contribute to the prostate cancer development. The enhanced XAF1 expression by somatostatin indicates a promising strategy for prostate cancer therapy. Background Prostate cancer is the most common cancer and the leading cause of cancer death among men in the United States and Europe 1 2 . It was estimated that approximately 186 320 new cases and 28 660 prostate cancer-related deaths occurred in the US in 2008 1 . Although epidemiological studies showed that the incidence of prostate cancer in Asians is much lower than that in African-Americans 3 the