tailieunhanh - Báo cáo hóa học: " In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter"

Tham khảo tài liệu 'báo cáo hóa học: " in vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | BioMed Central Virology Journal Research Open Access In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter Agustin Luz-Madrigal1 Carmen Clapp2 Jorge Aranda2 and Luis Vaca 1 Address 1Departamento de Biología Celular Instituto de Fisiología Celular Universidad Nacional Autónoma de México UNAM Ciudad Universitaria México . 04510 México and 2Instituto de Neurobiología UNAM-Juriquilla Querétaro Qro México 76001 México Email Agustín Luz-Madrigal - aluz@ Carmen Clapp - clapp@ Jorge Aranda - snm327@ Luis Vaca - lvaca@ Corresponding author Published 18 September 2007 Received 21 June 2007 Accepted 18 September 2007 Virology Journal 2007 4 88 doi 1743-422X-4-88 This article is available from http content 4 1 88 2007 Agustín et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Endothelial cells are a target for gene therapy because they are implicated in a number of vascular diseases. Recombinant baculovirus have emerged as novel gene delivery vectors. However there is no information available concerning the use of endothelial-specific promoters in the context of the baculovirus genome. In the present study we have generated a recombinant baculovirus containing the human flt-1 promoter BacFLT-GFP driving the expression of the green fluorescent protein. Transcriptional gene targeting was analyzed in vitro in different mammalian cell lines and in vivo in adult rat retinal vasculature. Results BacFLT-GFP evoked the highest levels of expression in the endothelial cell line BUVEC-E6E7-1 similar to those reached by recombinant baculovirus carrying the CMV promoter 112 .