tailieunhanh - báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling | Abdel aziz et al. Journal of Experimental Clinical Cancer Research 2011 30 49 http content 30 1 49 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats Possible Role of Wnt Signaling Mohamed T Abdel aziz 1 Mohamed F El Asmar2 Hazem M Atta1 Soheir Mahfouz3 Hanan H Fouad1 1 11 1 1 Nagwa K Roshdy Laila A Rashed Dina Sabry Amira A Hassouna and Fatma M Taha Abstract Background The present study was conducted to evaluate the tumor suppressive effects of bone marrow derived mesenchymal stem cells MSCs in an experimental hepatocellular carcinoma HCC model in rats and to investigate the possible role of Wnt signaling in hepato-carcinogenesis. Methods Ninety rats were included in the study and were divided equally into Control group rats which received MSCs only rats which received MSCs vehicle only HCC group induced by diethylnitroseamine DENA and CCl4 rats which received MSCs after HCC induction rats which received MSCs before HCC induction. Histopathological examination and gene expression of Wnt signaling target genes by real time reverse transcription-polymerase chain reaction RT-PCR in rat liver tissue in addition to serum levels of ALT AST and alpha fetoprotein were performed in all groups. Results Histopathological examination of liver tissue from animals which received DENA-CCl4 only revealed the presence of anaplastic carcinoma cells and macro-regenerative nodules type II with foci of large and small cell dysplasia. Administration of MSCs into rats after induction of experimental HCC improved the histopathological picture which showed minimal liver cell damage reversible changes areas of cell drop out filled with stem cells. Gene expression in rat liver tissue demonstrated that MSCs downregulated b-catenin proliferating cell nuclear antigen PCNA cyclin D and survivin genes expression in liver tissues after HCC induction. Amelioration of the liver .