tailieunhanh - báo cáo khoa học: " An imbalance in progenitor cell populations reflects tumour progression in breast cancer primary culture models"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: An imbalance in progenitor cell populations reflects tumour progression in breast cancer primary culture models | Donatello et al. Journal of Experimental Clinical Cancer Research 2011 30 45 http content 30 1 45 Journal of Experimental Clinical Cancer Research RESEARCH Open Access An imbalance in progenitor cell populations reflects tumour progression in breast cancer primary culture models 1 1 2 3 5 Simona Donatello Lance Hudson David C Cottell Alfonso Blanco Igor Aurrekoetxea 1 Martin J Shelly Pcutor A rwn n6 R l nll I . Ci irir n Cfnlznc Arnnld mix I lill1 nH Ann 1 1 I Innlcinc1 reter A Deivan Malcolm R Kell Mauiice Stokes Ainold DK Hill and Ann M Hopkins Abstract Background Many factors influence breast cancer progression including the ability of progenitor cells to sustain or increase net tumour cell numbers. Our aim was to define whether alterations in putative progenitor populations could predict clinicopathological factors of prognostic importance for cancer progression. Methods Primary cultures were established from human breast tumour and adjacent non-tumour tissue. Putative progenitor cell populations were isolated based on co-expression or concomitant absence of the epithelial and myoepithelial markers EPCAM and CALLA respectively. Results Significant reductions in cellular senescence were observed in tumour versus non-tumour cultures accompanied by a stepwise increase in proliferation senescence ratios. A novel correlation between tumour aggressiveness and an imbalance of putative progenitor subpopulations was also observed. Specifically an increased double-negative DN to double-positive DP ratio distinguished aggressive tumours of high grade estrogen receptor-negativity or HER2-positivity. The DN DP ratio was also higher in malignant MDA-MB-231 cells relative to non-tumourogenic MCF-10A cells. Ultrastructural analysis of the DN subpopulation in an invasive tumour culture revealed enrichment in lipofuscin bodies markers of ageing or senescent cells. Conclusions Our results suggest that an imbalance in tumour progenitor subpopulations imbalances .