tailieunhanh - báo cáo khoa học: " Silibinin induces apoptosis via calpain-dependent AIF nuclear translocation in U87MG human glioma cell death"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Silibinin induces apoptosis via calpain-dependent AIF nuclear translocation in U87MG human glioma cell death | Jeong et al. Journal of Experimental Clinical Cancer Research 2011 30 44 http content 30 1 44 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Silibinin induces apoptosis via calpain-dependent AIF nuclear translocation in U87MG human glioma cell death Ji C Jeong1 Won Y Shin1 Thae H Kim2 Chae H Kwon2 Jae H Kim2 Yong K Kim2 and Ki H Kim3 Abstract Background Silibinin a natural polyphenolic flavonoid has been reported to induce cell death in various cancer cell types. However the molecular mechanism is not clearly defined. Our previous study showed that silibinin induces glioma cell death and its effect was effectively prevented by calpain inhibitor. The present study was therefore undertaken to examine the role of calpain in the silibinin-induced glioma cell death. Methods U87MG cells were grown on well tissue culture plates and cell viability was measured by MTT assay. ROS generation and Aym were estimated using the fluorescence dyes. PKC activation and Bax expression were measured by Western blot analysis. AIF nuclear translocation was determined by Western blot and immunocytochemistry. Results Silibinin induced activation of calpain which was blocked by EGTA and the calpain inhibitor Z-Leu-Leu-CHO. Silibinin caused ROS generation and its effect was inhibited by calpain inhibitor the general PKC inhibitor GF 109203X the specific PKCS inhibitor rottlerin and catalase. Silibinin-induce cell death was blocked by calpain inhibitor and PKC inhibitors. Silibinin-induced PKCS activation and disruption of Aym were prevented by the calpain inhibitor. Silibinin induced AIF nuclear translocation and its effect was prevented by calpain inhibitor. Transfection of vector expressing microRNA of AIF prevented the silibinin-induced cell death. Conclusions Silibinin induces apoptotic cell death through a calpain-dependent mechanism involving PKC ROS and AIF nuclear translocation in U87MG human glioma cells. Background Glioblastoma is the most .