tailieunhanh - báo cáo khoa học: "Promising cytotoxic activity profile of fermented wheat germ extract (Avemar®) in human cancer cell lines"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Promising cytotoxic activity profile of fermented wheat germ extract (Avemar®) in human cancer cell lines | Mueller et al. Journal of Experimental Clinical Cancer Research 2011 30 42 http content 30 1 42 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Promising cytotoxic activity profile of fermented wheat germ extract Avemar in human cancer cell lines Thomas Mueller Karin Jordan and Wieland Voigt Abstract Fermented wheat germ extract FWGE is currently used as nutrition supplement for cancer patients. Limited recent data suggest antiproliferative antimetastatic and immunological effects which were at least in part exerted by two quinones 2-methoxy benzoquinone and 2 6-dimethoxybenzquinone as ingredients of FWGE. These activity data prompted us to further evaluate the in vitro antiproliferative activity of FWGE alone or in combination with the commonly used cytotoxic drugs 5-FU oxaliplatin or irinotecan in a broad spectrum of human tumor cell lines. We used the sulforhodamine B assay to determine dose response relationships and IC50-values were calculated using the Hill equation. Drug interaction of simultaneous and sequential drug exposure was estimated using the model of Drewinko and potential clinical activity was assessed by the model of relative antitumor activity RAA . Apoptosis was detected by DNA gel electrophoresis. FWGE induced apoptosis and exerted significant antitumor activity in a broad spectrum of 32 human cancer cell lines. The highest activity was found in neuroblastoma cell lines with an average IC50 of mg ml. Furthermore IC50-range was very narrow ranging from mg ml to mg ml in 8 colon cancer cell lines. At combination experiments in colon cancer cell lines when FWGE was simultaneously applied with either 5-FU oxaliplatin or irinotecan we observed additive to synergistic drug interaction particularly for 5-FU. At sequential drug exposure with 5-FU and FWGE the observed synergism was abolished. Taken together FWGE exerts significant antitumor activity in our tumor model. Simultaneous drug exposure with .