tailieunhanh - báo cáo khoa học: " Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells | Journal of Experimental Clinical Cancer Research Weiss-Steider et al. Journal of Experimental Clinical Cancer Research 2011 30 37 http content 30 1 37 RESEARCH Open Access Expression of MICA MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells Benny Weiss-Steider Isabel Soto-Cruz Christian A Martinez-Campos and Jorge Flavio Mendoza-Rincon Abstract Background Cancer cells are known to secrete the stress molecules MICA and MICB that activate cytotoxicity by lymphocytes and NK cells through their NKG2D receptor as a mechanism of immunological defense. This work was undertaken to evaluate if cancer cells can also express this receptor as a possible mechanisms of depletion of MIC molecules and thus interfere with their immune recognition. Methods Myelomonocytic leukemic TPH-1 and U-937 and cervical cancer CALO and INBL cell lines were evaluated by Western Blot ELISA flow cytometry and immunocytochemistry to evaluate their capacity to express and secrete MICA and MICB and to be induced to proliferate by these molecules as well as to express their receptor NKG2D. Statistical analysis was performed by two-way ANOVA for time course analysis and Student s t-test for comparison between groups. Values were considered significantly different if p . Results THP-1 and U-937 produce and secrete the stress MICA and MICB as shown by Western Blot of lysed cells and by ELISA of their conditioned media. By Western Blot and flow cytometry we found that these cells also express the receptor NKG2D. When THP-1 and U-937 were cultured with recombinant MICA and MICB they exhibited a dose dependent induction for their proliferation. CALO and INBL also produce MICA and MICB and were induced to proliferate by these stress molecules. By Western Blot flow cytometry and immunocytochemistry we also found that these cells express NKG2D. Conclusions Our novel results that tumor cells can simultaneously secrete MIC molecules and express their receptor and to be .