tailieunhanh - báo cáo hóa học:" Fourier-transform infrared anisotropy in cross and parallel sections of tendon and articular cartilage"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Fourier-transform infrared anisotropy in cross and parallel sections of tendon and articular cartilage | Journal of Orthopaedic Surgery and Research BioMed Central Open Access Research article Fourier-transform infrared anisotropy in cross and parallel sections of tendon and articular cartilage Nagarajan Ramakrishnan Yang Xia and Aruna Bidthanapally Address Department of Physics and Center for Biomedical Research Oakland University Rochester MI 48309 USA Email Nagarajan Ramakrishnan - ramakris@ Yang Xia - xia@ Aruna Bidthanapally - bidthana@ Corresponding author Published 6 October 2008 Received 6 May 2008 Journal of Orthopaedic Surgery and Research 2008 3 48 doi 1749-799X-3-48 Accepted 6 October 2008 This article is available from http content 3 1 48 2008 Ramakrishnan et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Fourier Transform Infrared Imaging FTIRI is used to investigate the amide anisotropies at different surfaces of a three-dimensional cartilage or tendon block. With the change in the polarization state of the incident infrared light the resulting anisotropic behavior of the tissue structure is described here. Methods Thin sections 6 pm thick were obtained from three different surfaces of the canine tissue blocks and imaged at pm pixel resolution. For each section infrared imaging experiments were repeated thirteen times with the identical parameters except a 15 increment of the analyzer s angle in the 0 - 180 angular space. The anisotropies of amide I and amide II components were studied in order to probe the orientation of the collagen fibrils at different tissue surfaces. Results For tendon the anisotropy of amide I and amide II components in parallel sections is comparable to that of regular sections and tendon s cross .