tailieunhanh - báo cáo khoa học: "Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway | Xu et al. Journal of Experimental Clinical Cancer Research 2011 30 33 http content 30 1 33 Journal of Experimental Clinical Cancer Research RESEARCH Open Access Osthole induces G2 M arrest and apoptosis in lung cancer A549 cells by modulating PI3K Akt pathway Xiaoman Xu1 Yi Zhang2 Dan Qu1 Tingshu Jiang1 and Shengqi Li1 Abstract Background To explore the effects of Osthole on the proliferation cell cycle and apoptosis of human lung cancer A549 cells. Methods Human lung cancer A549 cells were treated with Osthole at different concentrations. Cell proliferation was measured using the MTT assay. Cell cycle was evaluated using DNA flow cytometry analysis. Induction of apoptosis was determined by flow cytometry and fluorescent microscopy. The expressions of Cyclin B1 p-Cdc2 Bcl-2 Bax t-Akt and p-Akt were evaluated by Western blotting. Results Osthole inhibited the growth of human lung cancer A549 cells by inducing G2 M arrest and apoptosis. Western blotting demonstrated that Osthole down-regulated the expressions of Cyclin B1 p-Cdc2 and Bcl-2 and up-regulated the expressions of Bax in A549 cells. Inhibition of PI3K Akt signaling pathway was also observed after treating A549 cells with Osthole. Conclusions Our findings suggest that Osthole may have a therapeutic application in the treatment of human lung cancer. Background Lung cancer is the leading cause of cancer-related death in the world and non-small cell lung cancer accounts for approximately 80 of all cases 1 2 . Despite advances in diagnostic and therapeutic the overall 5-year survival rate in many countries is generally less than 15 3 . In order to improve the survival rate intensive efforts have been made to find new anticancer agents and many attentions have been drawn to herbal medicines owing to their wide range of biological activities low toxicity and side effects 4-6 . Osthole 7-methoxy-8- 3-methyl-2-butenyl coumarin Figure 1 is an active constituent of Cnidium monnieri L. Cusson has been .