tailieunhanh - Báo cáo khoa học: " What can nuclear microsatellites tell us about maritime pine genetic resources conservation and provenance certification strategies"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: What can nuclear microsatellites tell us about maritime pine genetic resources conservation and provenance certification strategies?. | Ann. For. Sci. 59 2002 699-708 INRA EDP Sciences 2002 DOI forest 2002058 699 Original article What can nuclear microsatellites tell us about maritime pine genetic resources conservation and provenance certification strategies Jérémy Derorya Stéphanie Mariettea b Santiago C. Gonzaléz-Martínezc David Chagnéa TAinl A Ịo Ì11 f a QJ1 1 TớOĩì T t o t a IdvoHr Ai 0 Pỡrovnd A fovio A Díhoỉrrt Deiphine Madur Sophie Gerber Jean Brach Francois Persyn Maria M. Ribeiro and Christophe Plomiona aINRA Équipe de Génétique et Amelioration des Arbres Forestiers 69 route d Arcachon 33612 Cestas Cedex France DCemagref Domaine des Barres 45290 Nogent-sur-Vernisson France cCIFOR-INIA Department of Breeding and Biotechnology . Box 8111 28080 Madrid Spain d Services Espaces Verts 62520 Le Touquet France eEscola Superior Agrária Dep de Silvicultura e Recursos Naturais Quinta da Senhora de Mércules Apdo 119 6001-909 Castelo Branco Portugal Received 16 August 2001 accepted 13 March 2002 Abstract - Maritime pine Pinus pinaster Ait. is the first conifer used for reforestation in France and now covers million ha of the Iberian Peninsula. In order to preserve the genetic resources of this economically and ecologically important species prior knowledge of the distribution of genetic diversity is needed. In this paper a genetic diversity study was performed using nuclear simple sequence repeats SSRs ormicrosatel-lites . Classical parameters of diversity allelic richness and heterozygosity and differentiation were estimated for 47 populations of P. pinaster. Most of the populations 40 were collected in France six populations were also collected in the Iberian Peninsula and one Moroccan population was also included in the study. The population genetic parameters indicated that some populations should be a focus of conservation efforts higher level of diversity higher allelic richness and presence of rare alleles . A diagnostic test for sample origin was developed to distinguish .